Determination of True Electrophoretic Mobility
When the measurement of electrophoresis is actually taken, an electroosmotic current is generated in the cell due to an electric charge on the cell wall. With a negatively charged cell wall, the electroosmotic flow phenomenon causes the positively charged ions and particles to gather together by the cell walls. The solution located by the cell walls migrates toward the negative electrode during electrophoresis. The solution located in the cell center moves in the opposite direction (toward the positive electrode) to compensate for the flow by the cell walls. Therefore, an electroosmotic flow is created during electrophoresis. The NanoPlus is designed to measure electrophoretic mobility at several points in the cell to obtain a position (i.e. static) not influenced by electroosmotic flow. As a result, the instrument can calculate and accurately measure electrophoretic mobility, even if the electroosmotic profile of the system is asymmetrical due to adsorption or sedimentation of the sample.
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